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ELISA, or enzyme-linked immunosorbent assay, is a plate-based immunoassay used to detect and quantify biomolecules such as antibodies, proteins, hormones or peptides, as well as for the characterization of protein-protein and protein-nucleic acid interactions.
Power Styramide™ Signal Amplification (PSA™) is a novel enzymatic amplification method used to detect low-abundance targets in cells and tissues. By combining the superior brightness and photostability of iFluor™ dyes with poly-HRP mediated PSA™ imaging, generate bright fluorescence signals with significantly higher precision and sensitivity (more than 100-fold greater) than conventional immunohistochemistry, immunocytochemistry and in situ hybridization techniques.
Primary antibodies, also known as immunoglobulins (Ig), are large Y-shaped proteins produced and used by the immune system to identify specific biomolecules, called antigens. The recognition between an antibody and an antigen is usually described as a lock-key mechanism: the paratope (analogous to a lock) on each tip of the “Y” of an antibody precisely binds to one particular epitope (analogous to a key). The degree to which an antibody’s binding site will interact with an antigen is called its affinity.
Secondary antibody conjugates are important tools in biochemical and cell-based studies. Enzyme-labeled secondary antibodies, such as HRP secondaries, have high catalytic turnover rates and are routinely used in various assays for their rapid and robust signal generation. HRP secondary antibodies have been used extensively in enzyme-linked immunosorbent assays (ELISA), lateral flow assays (LFA), immunohistochemistry (IHC) and Western blot for the diagnosis of infectious diseases. Secondary antibodies conjugated to fluorescent dyes provide brighter signals and multiplexing capabilities in cell analysis and protein analysis applications including immunofluorescence microscopy and cell imaging.
As clinical diagnostic and surveillance tools, serological assays such as the enzyme-linked immunosorbent assay (ELISA) and the lateral flow immunoassay (LFIA) have been extensively used in the detection and identification of infectious diseases. They achieve this aim through the use of highly specific recombinant antigens, or fusion proteins, to detect serum antibodies, which are used by the immune system to neutralize pathogenic bacteria, viruses or other microorganisms that cause disease. Serological assays have been successfully used in the detection of hepatitis viruses, the ebola virus, HIV and influenza.
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