Cell Analysis


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Cell Cycle and Proliferation

Live & Fixed Cell Cycle Assays, Cell Tracking & Proliferation Indicators

Cell proliferation is described as an increase in cell number in any given tissue in vivo or cell culture plates in vitro. Cells proliferate themselves, in their favourable conditions, by a process known as Cell Cycle. Cell cycle is defined as an event that takes place in cells, where one cell divides itself into two daughter cells. 

Cell Metabolism & Cell Signaling

Cell metabolism, Cell Signaling, Biomolecule Quantification, Enzyme Cofactor Quantification, Oxidative stress

The Amplite™ product series is composed of enzyme-based substrates and assays optimized for measuring horseradish peroxidase (HRP), luciferase, phosphodiesterases, proteases, histone deacetylase (HDAC) and cell signaling molecules such as NAD/NADH, NADP/NADPH, etc. The series includes assay kits spanning essentially all aspects of cellular and physiological activity, and is compatible with other AAT Bioquest products as well as classic materials.

Cell Structures and Organelles

Fluorescence Microscopy, Organelle Labeling Probes & Kits, Actin, ER, Golgi, Lysosomes, Mitochondria, Nucleus, Plasma Membrane

Organelles are specialized structures that play a critical part in cellular function. Nearly all cellular activities are facilitated by organelles working either singly or in concert, ranging from gene expression and cell signaling to energy production and apoptosis. To fully understand cellular behavior, researchers utilize organelle-selective probes to detect, visualize and track organelle activity, including the biochemical mechanisms that govern them. These probes are ideal for co-localization studies serving as excellent counter-stains for identifying location-specific proteins and targets of interest within the cell.

Cell Viability

Exclusion Assays, Reduction Assays, Fluorescent Esterase Substrates

Cell viability often corresponds with cell health and the ability of a cell population to survive and function successfully. Measurements for cell viability can be used to correlate cell behavior with the number of viable cells in a population, optimize the growth conditions of cell populations maintained in culture, test toxicology, or assess the efficacy of potential drug candidates. The parameters which define cell viability can be diverse, ranging from the integrity of cell membranes or the activity of intracellular enzymes to the membrane potential of mitochondria or the redox potential of a cell population

Membrane Potential and Channels

Slow & Fast Membrane Potential Probes, FLIPR® Membrane Potential Assays

Membrane potential is the difference in voltage between the interior and exterior of a cell. The membrane potential allows a cell to function as a battery, providing power to operate a variety of molecular devices embedded in the membrane. In electrically excitable cells such as neurons, membrane potential is used for transmitting signals between different parts of a cell. Opening or closing of ion channels at one point in the membrane produces a local change in the membrane potential, which causes an electric current to flow rapidly to other points in the membrane. Ion channels have been identified as important drug discovery targets. 

Mitochondrial Membrane Potential

JC-1 & JC-10™ Membrane Potential Probes, Flow Cytometry

The mitochondrial membrane potential (ΔΨm), generated by the electron transport chain, is a key parameter necessary for healthy mitochondrial functioning. Together with the proton gradient, it generates the driving force behind mitochondrial ATP synthesis. It plays a key role in mitochondrial homeostasis through selective elimination of dysfunctional mitochondria, and is an essential component of mitochondrial calcium homeostasis.

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