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Fluorescence is the result of a three-stage process that occurs in certain molecules (generally polyaromatic hydrocarbons or heterocycles) called fluorophores or fluorescent dyes. Fluorescent probes enable researchers to detect particular components of complex biomolecular assemblies (including live cells) with exquisite sensitivity and selectivity. Reactive fluorescent dyes are widely used to modify amino acids, peptides, proteins (in particular, antibodies), oligonucleotides, nucleic acids, carbohydrates and other biological molecules.
AAT Bioquest iFluor™ dyes are a series of highly water-soluble fluorescent dyes for covalent labeling of biomolecules, such as proteins and antibodies. Available in a variety of excitation-emission profiles, spanning the UV-visible to near-infrared spectrum, there is an iFluor™ dye to match any instrument set-up and many combinations for multiplex detection. Conjugates made with iFluor™ dyes exhibit superior brightness and photostability, outperforming Alexa Fluor® conjugates and other spectrally similar conjugates. Use iFluor™ products for all your immunofluorescence assays, cell imaging and flow cytometric applications.
Indocyanine green (ICG) is a negatively charged, tricarbocyanine dye that is widely exploited for its low toxicity and infrared fluorescence emission. ICG’s minimal toxicity is attributed to its strict binding with plasma proteins. This confines it to the vascular compartment where it is rapidly metabolized by the liver and excreted exclusively by the liver and bile ducts. ICG and ICG derivatives can be used as in vivo molecular imaging probes. Their infrared fluorescence emission penetrates tissues several millimeters to facilitate deep tissue and small animal in vivo imaging. ICG is used in medical diagnostics for determining cardiac output, hepatic function, and liver blood flow and for ophthalmic angiography. In addition, ICG has been conjugated to several antibodies for cancer cell and tumor targeting.
mFluor™ dyes are a series of superior fluorescent labeling dyes that span the UV to near-infrared (NIR) spectrum. Developed exclusively by AAT Bioquest, mFluor™ dyes are designed to expand the multicolor capabilities of flow cytometers, making multicolor panel design easier.
Phycoerythrin (PE) and allophycocyanin (APC), which belong to the phycobiliprotein family, exhibit intensely bright fluorescence due to their exceptionally high quantum yields and extinction coefficients. Synthesis of these fluorophores to molecules having biological specificity – antibodies, protein A, or streptavidin – form extraordinarily luminescent probes that can be used in fluorescence-based detection applications which require high sensitivity but not photostability, primarily flow cytometry, FACS and immunophenotyping.
Tide Fluor™ dyes are optimized as building blocks for developing FRET oligonucleotides and peptides. Our Tide Fluor™ dyes have stronger fluorescence and higher photostability than the classic labeling dyes such as coumarins, fluoresceins, rhodamines and cyanines. They are the best affordable fluorescent dyes for labeling peptides and oligonucleotides without sacrificing performance.
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