Hoechst 33342 *Ultrapure Grade*


Unit Size: 100mg
Price:
$236

Overview 


CAS: 875756-97-1             

Molecular weight: 561.93

Emission (nm): 352        

Excitation (nm): 454 

The Hoechst stains are a family of fluorescent stains for labeling DNA in fluorescence microscopy. Because these fluorescent stains label DNA, they are also commonly used to visualize nuclei and mitochondria. Two of these closely related bis-benzimides are commonly used: Hoechst 33258 and Hoechst 33342. Both dyes are excited by ultraviolet light at around 350 nm, and both emit blue/cyan fluorescence light around an emission maximum at 461 nm. The Hoechst stains may be used on live or fixed cells, and are often used as a substitute for another nucleic acid stain, DAPI. The key difference between them is that the additional ethyl group of Hoechst 33342 renders it more lipophilic, and thus more able to cross intact cell membranes. In some applications, Hoechst 33258 is significantly less permeant. These dyes can also be used to detect the contents of a sample DNA by plotting a standard emission-to-content curve.

Platform

Fluorescence Microscope

Excitation (nm): 350

Emission (nm): 461       

Recommended Plate: Black wall/clear bottom

 

Citations

Carboxylated branched poly (β-amino ester) nanoparticles enable robust cytosolic protein delivery and CRISPR-Cas9 gene editing
Authors: Rui, Yuan and Wilson, David R and Choi, John and Varanasi, Mahita and S and ers, Katie and Karlsson, Johan and Lim, Michael and Green, Jordan J
Journal: Science Advances (2019): eaay3255

From Energy Deposition of Ionizing Radiation to Cell Damage Signaling: Benchmarking Simulations by Measured Yields of Initial DNA Damage after Ion Microbeam Irradiation
Authors: Gonon, G{\'e}raldine and Villagrasa, Carmen and Voisin, Pascale and Meylan, Sylvain and Bueno, Marta and Benadjaoud, Mohamed Amine and Tang, Nicolas and Langner, Frank and Rabus, Hans and Barquinero, Joan-Francesc and others
Journal: Radiation research (2019): 566--584

Cell electrofusion to improve efficacy and thermotolerance of the entomopathogenic fungus, Beauveria bassiana
Authors: Davari, Agrin and Skinner, Margaret and Parker, Bruce L
Journal: Journal of applied microbiology (2018)

Polyethylene glycol and octaarginine n dual-functionalized nano-graphene oxide: An optimization for efficient nucleic acids delivery
Authors: Imani, Rana and Prakash, Satya and Vali, Hojatollah and Faghihi, Shahab
Journal: Biomaterials Science (2018)

Quanyuan Wan1, Chunrong Yang2, Youliang Rao1, Zhiwei Liao1 and Jianguo Su1
Authors: Kishore, Uday and Tan, Yunhao and Tamassia, Nicola and Su, Jianguo and Wan, Q and Yang, C and Rao, Y and Liao, Z
Journal: Macromolecular Structure Underlying Recognition in Innate Immunity (2018): 12135


References

 

Grafting of Ring-Opened Cyclopropylamine thin films on Silicon (100) Hydride via UV Photoionization
Authors: Tung, Joline and Ching, Jing Yuan and Ng, Yoke Mooi and Tew, Lih Shin and Khung, Yit Lung
Journal: ACS Applied Materials & Interfaces (2017)

MDA5 Induces a Stronger Interferon Response than RIG-I to GCRV Infection through a Mechanism Involving the Phosphorylation and Dimerization of IRF3 and IRF7 in CIK Cells
Authors: Wan, Quanyuan and Yang, Chunrong and Rao, Youliang and Liao, Zhiwei and Su, Jianguo
Journal: Frontiers in Immunology (2017)

Dual-functionalized graphene oxide for enhanced siRNA delivery to breast cancer cells
Authors: Imani, Rana and Shao, Wei and Taherkhani, Samira and Emami, Shahriar Hojjati and Prakash, Satya and Faghihi, Shahab
Journal: Colloids and Surfaces B: Biointerfaces (2016): 315--325

A C-terminal acidic domain regulates degradation of the transcriptional coactivator Bob1
Authors: Lindner, John M and Wong, Christina SF and Möller, Andreas and Nielsen, Peter J
Journal: Molecular and cellular biology (2013): 4628--4640

Fatty acid synthase and its mRNA concentrations are decreased at different times following Hoechst 33342-induced apoptosis in BC3H-1 myocytes
Authors: Zhang X, Kiechle FL.
Journal: Ann Clin Lab Sci (2006): 185

Usefulness of a triple fluorochrome combination Merocyanine 540/Yo-Pro 1/Hoechst 33342 in assessing membrane stability of viable frozen-thawed spermatozoa from Estonian Holstein AI bulls
Authors: Hallap T, Nagy S, Jaakma U, Johannisson A, Rodriguez-Martinez H.
Journal: Theriogenology (2006): 1122

Acid-base and electronic structure-dependent properties of Hoechst 33342
Authors: Aleman C, Namba AM, Casanovas J.
Journal: J Biomol Struct Dyn (2005): 29

An in vitro study of Hoechst 33342 redistribution and its effects on cell viability
Authors: Mohorko N, Kregar-Velikonja N, Repovs G, Gorensek M, Bresjanac M.
Journal: Hum Exp Toxicol (2005): 573

Evidence for a qualitative hierarchy within the Hoechst-33342 'side population' (SP) of murine bone marrow cells
Authors: Robinson SN, Seina SM, Gohr JC, Kuszynski CA, Sharp JG.
Journal: Bone Marrow Transplant (2005): 807

Flow cytometric characterization of viable meiotic and postmeiotic cells by Hoechst 33342 in mouse spermatogenesis
Authors: Bastos H, Lassalle B, Chicheportiche A, Riou L, Testart J, Allem and I, Fouchet P.
Journal: Cytometry A (2005): 40

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